Table 5.

The expression variations of 17 unigenes from transcriptome and qRT-PCR analysis

Unigene ID	LD (transcriptome)		CK		qRT-PCR(LD)		HD (transcriptome)		qRT-PCR(HD)
	Log2Ratio (CK-LD)	Fold change	Means	SEM	Means	SEM	Log2Ratio (CK-HD)	Fold change	Means	SEM
CL2087-1	4.98	31.559	1.000	0.075	7.724	1.223**	2.25	4.757	6.236	0.870**
U5019	4.45	21.857	1.000	0.039	3.311	0.498**	3.90	14.929	5.557	1.144**
CL2702-3	4.06	16.679	1.000	0.112	10.460	2.250**	2.60	6.063	2.289	0.295**
U33624	3.74	13.361	1.000	0.085	5.802	1.416**	2.34	5.063	3.126	0.533**
CL2319-1	3.32	9.987	1.000	0.058	1.265	0.181	4.10	17.148	0.983	0.112
U13389	2.81	7.013	1.000	0.136	1.744	0.322*	2.43	5.389	1.138	0.116
CL2611-1	2.77	6.821	1.000	0.102	4.123	0.710**	2.34	5.063	2.790	0.428**
U21296	2.37	5.169	1.000	0.071	0.112	0.042**	2.24	4.724	0.308	0.060**
U29431	1.12	2.173	1.000	0.065	0.470	0.086**	0.59	1.505	1.117	0.085
CL4026-1	−7.30	0.006	1.000	0.032	0.029	0.007**	−1.92	0.264	0.359	0.048**
U6027	−3.69	0.077	1.000	0.114	0.016	0.004**	−1.42	0.374	0.812	0.071*
CL2853-2	−3.50	0.088	1.000	0.070	0.325	0.042**	−2.64	0.160	0.283	0.054**
U31578	−1.81	0.285	1.000	0.072	1.144	0.118	−1.21	0.432	0.952	0.117
U31293	−1.71	0.306	1.000	0.056	1.113	0.095	−2.52	0.174	0.864	0.093
CL181-1	−1.30	0.406	1.000	0.064	2.029	0.530**	−0.72	0.607	1.090	0.125
U38505	0.98	1.972	1.000	0.103	0.403	0.061**	−2.92	0.132	0.272	0.046**
CL3168-1	0.50	1.414	1.000	0.085	0.957	0.122	1.20	2.297	0.886	0.069

* Significantly different at 0.05 level; **significantly different at 0.01 level.