Figure 6.

Capsid surface of AAV8. (A) Exterior capsid surface density image of AAV8 with the two lysine residues mutated colored and labeled (K547 [equivalent to AAV2 K544] in red and K569 in dark green). Residue K547 is in bold underline. The partially buried nature of K569 is evident in this image. The AU is depicted as in Fig. 5. This image was generated using the PyMOL program.²⁶ (B) Stereographic Roadmap²⁵ of the AAV8 virus capsid showing a zoomed-in image of the viral AU [as described in (A)] viewed down the icosahedral twofold axis. The surface representation of K547 is shown in red. K569 is not surface seen in this 2D representation of the capsid surface. As in Fig. 5B, the black lines indicate the boundaries between the residues. The icosahedral axes are indicated as in Fig. 5A.