Table 1.

Primers Used for Generating DBD Hemi-Driver by Extension PCRs

Round	Primers (5′-3′)
1	F: GCTACGGCCCCCTGGGCGGCGGCAAGGCTAGCGGAGGAGGCGGCGGAGGCGGAGGCGGAGGCACTAGTATGAAACTGCTCTCATCCATCG
	R: ggggaccactttgtacaagaaagctgggtTTACGAAACAGTCAGCTGTCTCTG
2	F: CCGAGGTGGCCGAGCTGGAGCAGGAGGTGCAGCGCCTGGAGAACGAGGTGAGCCAGTACGAGACCCGCTACGGCCCCCTGGGCGGCGGC
	R: same as Round 1
3	F: GCCGCCACCATGCTGGAGATCCGCGCCGCCTTCCTGCGCCAGCGCAACACCGCCCTGCGCACCGAGGTGGCCGAGCTGGAGCAGG
	R: same as Round 1
4	F: ggggacaagtttgtacaaaaaagcaggctGCCGCCACCATGCTGGAGATCCG
	R: same as Round 1

Italics denote KalTA4's DBD coding sequence, bold denotes the ‘Zip’ sequence, and underlined is the ‘gly’ sequence.

DBD, DNA-binding domain; PCR, polymerase chain reaction.