Table 2.

Primers Used for Generating TA4 Hemi-Driver by Extension PCRs

Round	Primers (5′-3′)
1	F: CCACCATGGATAAA-ATGGCTCCAAAGAAGAAGCGTAAGGTA-CGGCCGACGCCCTGGACG
	R: CCAGGAAGGCGGCCTCGATCTCCAGACTAGTGCCTCCGCCTCCGCCTCCGCCGCCTCCTCCGCTAGCGAGGATGTCCAGGTCGTAGTCG
2	F: ggggacaagtttgtacaaaaaagcaggctGCCGCCACCATGGATAAA
	R: GCGCAGGCGCTGCACGCGCTGGCGCAGCTCGGCCACGCGGGTCTCCAGGGCGGTGTTCTCGCGCTCCAGGAAGGCGGCCTCGATCTCC
3	F: same as Round 2
	R: TTACTTGCCGCCGCCCAGGGGGCCGTAGCGGGTGCGGTTCTGGCTCACGCGGTTGCGCAGGCGCTGCACGCGCTGG
4	F: same as Round 2
	R: ggggaccactttgtacaagaaagctgggtTTACTTGCCGCCGCCCAGGGGGC

Italics denote the TA4 coding sequence, bold denotes the ‘Zip’ sequence, and underlined is the ‘gly’ sequence.