FIG. 2.

Quantification of H3K18ac/DNA signal intensities in embryos at the two-cell and blastocyst stages. (A) Staining of H3K18ac in embryos at the two-cell (left columns) and blastocyst stages (right columns). Each sample was counterstained with DAPI to visualize the DNA. Scale bars, 50 μm. (B) Labeling intensity was expressed relative to the IVF group (set as 1.0). Quantification of the H3K18ac/DNA ratio is represented as the mean ± standard error of the mean. Values with different superscripts differ significantly (p < 0.05). Experiments were replicated three times. In each replication, n = 10 per group.