. 2015 Dec 1;17(6):484–493. doi: 10.1089/cell.2015.0049

Table 2.

Effect of Different Concentrations of Recombinant Human BMP6 on the Subsequent Developmental Competence of Cloned Yak Embryos

Groups	No. oocytes	No. (mean ± SEM%) MII oocytes	No. (mean ± SEM%) ≥ two-cell embryos	No. (mean ± SEM%) blastocysts
0 ng/mL	242	160 (66.12 ± 2.04)^a	111 (69.40 ± 1.03)^a	33 (20.63 ± 1.32)^a
50 ng/mL	256	172 (67.18 ± 1.52)^ab	121 (70.34 ± 1.14)^a	38 (22.09 ± 1.14)^ab
100 ng/mL	238	174 (73.11 ± 1.38)^c	136 (78.16 ± 0.93)^b	45 (28.16 ± 1.67)^c
200 ng/mL	261	180 (69.73 ± 1.84)^b	132 (73.32 ± 0.88)^c	40 (22.22 ± 1.02)^ab

Six replicates were performed and approximately 40 cumulus–ooctye complexes (COCs) per replicate were used for each group. Numbers in parentheses were development rates (mean ± SEM%), whereas other numbers were total oocytes or embryos number of six replicates among groups.

Development rates of two-cell embryos and blastocysts were the percent of total metaphase II (MII) oocytes and were monitored at 48 and 168 h of culture, respectively (0 h being the time embryos were transferred to G1.5 medium).

^{^a,b,c}

Values with different superscripts within columns are significantly different from each other (p < 0.05).

BMP6, bone morphogenetic protein 6; SEM, standard error of the mean.