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. 2015 Dec 1;17(6):484–493. doi: 10.1089/cell.2015.0049

Table 4.

Characterization of Day 7 Yak Blastocysts From Different Treatments

Groups No. blastocysts Total no. of cells No. of TE cells No. of ICM cells No. of ICM cells:total cell ratio (%)
SCNT 42 85.24 ± 3.12a 65.65 ± 5.03a 19.59 ± 2.17a 22.93 ± 1.43a
SCNT + BMP6 36 103.36 ± 5.28b 71.16 ± 3.92b 32.20 ± 2.61b 31.21 ± 1.62b
IVF 41 105.83 ± 6.03b 72.69 ± 7.41b 33.14 ± 3.34b 31.29 ± 1.28b

The cell numbers in blastocysts were estimated by counting the total number of nuclei using 4′,6-diamidino-2-phenylindole (DAPI), whereas the number of trophectoderm nuclei was assessed using immunostaining for CDX2. The inner cell mass cell number was estimated as the total number of nuclei minus the number of trophectoderm nuclei. The data are shown as mean ± standard error of the mean (SEM). n refers to the total number of blastocysts analyzed.

a,b

Values with different superscripts within columns are significantly different from each other (p < 0.05).

TE, trophectoderm; ICM, inner cell mass; BMP6, bone morphogenetic protein 6; SCNT, somatic cell nuclear transfer, IVF, in vitro fertilization.