Table 4.
Characterization of Day 7 Yak Blastocysts From Different Treatments
Groups | No. blastocysts | Total no. of cells | No. of TE cells | No. of ICM cells | No. of ICM cells:total cell ratio (%) |
---|---|---|---|---|---|
SCNT | 42 | 85.24 ± 3.12a | 65.65 ± 5.03a | 19.59 ± 2.17a | 22.93 ± 1.43a |
SCNT + BMP6 | 36 | 103.36 ± 5.28b | 71.16 ± 3.92b | 32.20 ± 2.61b | 31.21 ± 1.62b |
IVF | 41 | 105.83 ± 6.03b | 72.69 ± 7.41b | 33.14 ± 3.34b | 31.29 ± 1.28b |
The cell numbers in blastocysts were estimated by counting the total number of nuclei using 4′,6-diamidino-2-phenylindole (DAPI), whereas the number of trophectoderm nuclei was assessed using immunostaining for CDX2. The inner cell mass cell number was estimated as the total number of nuclei minus the number of trophectoderm nuclei. The data are shown as mean ± standard error of the mean (SEM). n refers to the total number of blastocysts analyzed.
Values with different superscripts within columns are significantly different from each other (p < 0.05).
TE, trophectoderm; ICM, inner cell mass; BMP6, bone morphogenetic protein 6; SCNT, somatic cell nuclear transfer, IVF, in vitro fertilization.