FIG. 3.

AP staining and reprogramming efficiency calculation. (A) Representative image of AP-positive ESC-like colonies. (B) After selecting ESC-like colonies, the remaining cells treated with different lentiviruses were subjected to AP staining to calculate the efficiency of colony generation. A representative experiment is shown. (C) The number of AP-positive colonies generated from 5 × 10⁴ SKCs was counted to calculate the reprogramming efficiency. The results are shown as the mean ± standard deviation (SD) (error bars), n = 3, (**) p < 0.01.