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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Mol Biol. 2015;1276:101–131. doi: 10.1007/978-1-4939-2392-2_6

Table I.

Plasmids encoding RNAP subunits and σ70

Plasmid Relevant Characteristics Source
pET28a-NF-αNTDI-αNTDII KmR; ori-pBR322; PT7-FLAG-rpoA(1–235)-rpoA(1–235) (16)
pET21d-rpoB-CH6 ApR; ori-pBR322; PT7-rpoB-His6 (16)
pET21a-rpoC-CH6 ApR; ori-pBR322; PT7-rpoC-His6 (16)
pT7ω ApR; ori-pBR322; ori-fl; PT7-rpoZ (9)
pGEMD ApR; ori-pBR322; PT7-rpoD (18)