Skip to main content
. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: Science. 2015 Jan 30;347(6221):548–551. doi: 10.1126/science.aaa0986

Figure 3. TEFM increases processivity and stability of mtRNAP EC.

Figure 3

(A,B) TEFM increases the EC processivity. Transcription was performed using LSP templates lacking CSBII sequence to generate 500 nt (A) or 1000 nt (B) run-off products. Efficiency of synthesis of 500 nt RNA in the presence of TEFM is taken as 100%. (C) TEFM increases the EC stability. Complexes halted at +35 in the presence or absence of TEFM were incubated for the times indicated and chased with CTP. Numbers below indicate efficiency of a halted EC extension (%).