Table 1.
Loop-mediated isothermal amplification primer sequence of the amplified poly-protein coding gene to rapidly detect Wheat streak mosaic virus
| Set | Primer | Sequence (5′→3′) | Length (nt) | G+C (%) | Tm (°C) |
|---|---|---|---|---|---|
| WSMV_1 | F3 | GAGTTGGAGGAAAGGAAA | 18 | 44.4 | 49.0 |
| B3 | TCCGAAAACGGTAGAAAG | 18 | 44.4 | 49.3 | |
| FIP | GGACCAGAATATGTGAGTCGTATCTTTCACACGCAGAGT | 39 | - | - | |
| BIP | AGGAAGGTTACTCACCTTTGCGGAGTATAACTTGCAAGACG | 41 | - | - | |
|
| |||||
| WSMV_2 | F3 | TACGACTCACATATTCTGGT | 20 | 40.0 | 50.2 |
| B3 | GCCTATGAGTATAACTTGCA | 20 | 40.0 | 49.4 | |
| FIP | TCGGTCCTCGGAGATAGCGGAAGAAAGGTTGGAGAA | 36 | - | - | |
| BIP | ACAAAGGGAGTTCCTTGAGGATTTTCCGCAAAGGTGAGTAA | 41 | - | - | |