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Optimal loop-mediated isothermal amplification conditions
| Components | Condition (μl) | ||
|---|---|---|---|
|
| |||
| 1 | 2 | 3 | |
| Template | 1.5 | 1.5 | 1.5 |
| Bst. 10× reaction buffer | 2 | 2 | 2 |
| dNTP (10 mM) | 2 | 2 | 2 |
| Primers | |||
| F3 (10 pmol) | 0.6 | 0.6 | 0.6 |
| B3 (10 pmol) | 0.6 | 0.6 | 0.6 |
| FIP (10 pmol) | 1.4 | 1.4 | 1.4 |
| BIP (10 pmol) | 1.4 | 1.4 | 1.4 |
| Bst. polymerase (12 U) | 1.5 | 1.5 | 1.5 |
| ddH2O | 9 | 4 | 0 |
|
| |||
| Total (μl) | 20 | 15 | 11 |
