Figure 4.

Grouping-induced Courtship Behavior in fru^M null Males is Long-lasting. (A) Courtship indices of fru^LexA/fru^4-40 males, after being isolated (triangle) or grouped with wild-type females (square), to fresh wild-type females. X axis indicates number of days being isolated (triangle) or grouped (square) prior to testing. n = 12 for each. (B) Courtship indices of fru^LexA/fru^4-40 males, after being grouped with females for 8 days and then isolated from grouping for up to 7 days, to fresh females. X axis indicates number of days being isolated following the 8-day grouping and prior to testing. n = 12 for each. (C) Detail steps of courtship displayed by fru^LexA/fru^4-40 males after group-housing, compared with courtship by single-housed wild-type males. Arrows indicate tapping, wing extension, proboscis extension, abdomen bending and attempted copulation, respectively. n = 24 for each. (D) Latency of following and wing extension by fru^M null males and wild-type males. (E) Percentage of time displaying following and wing extension by fru^M null males and wild-type males. N.S., not significant; ***p < 0.001, two-sample t-test. Error bars indicate SEM. Please see Figure S2, Movie S4 and S5.