Table 1.

Oligonucleotides used in this study

Primer	Sequence	Description
ADEFP	5′-AACTGCAGATGGATAGCAAAACTGTTGG-3′	dsRNA construct. ADE2 nt 1–20; PstI site (underlined)
ADERG	5′-GAATAATTCTTCACCTTTAGACATCCCCAATGTGTAACAAGTCATCG-3′	dsRNA construct. yEGFP3 nt 24–1 antisense (underlined); ADE2 nt 414–392 antisense
ADEFH	5′-CCCAAGCTTATGGATAGCAAAACTGTTGG-3′	dsRNA construct. ADE2 nt 1–20; HindIII site (underlined)
ADERVH		dsRNA construct. ADE2 nt 414–392 antisense; HindIII site (underlined) and EcoRV site (double underlined)
ADEaG	5′-CGATGACTTGTTACACATTGGGGATGTCTAAAGGTGAAGAATTATTC-3′	dsRNA construct. ADE2 nt 392–414 sense (bold), yEGFP3 nt 1–20
GFPRH	5′-CCCAAGCTTCACCTTCACCGGAGACAGAAAATTTG-3′	dsRNA construct. yEGFP3 nt 100–73 antisense; HindIII site (underlined)
ADE2-35	5′-GGAGGTGGCCAATTAGGTCGTATG-3′	RT-PCR. ADE2 nt 35–58 sense
ACT237	5′-GAGAGTTGCTCCAGAAGAACATCC-3′	RT-PCR. ACT1 nt 943–966 sense
ACT994	5′-CCAAGATAGAACCACCAATCCAGAC-3′	RT-PCR. ACT1 nt 1700–1676 antisense
T7-A131	5′-CCAAGCTTCTAATACGACTCACTATAGGGTCTAGACGATCACGTTGATGGATCG-3′	ADE2 sense oligo nt 131–155 with T7 promoter (bold) and HindIII site (underlined)
A414	5′-CCCCAATGTGTAACAAGTCATCGAC-3′	ADE2 antisense oligo nt 414–389
A131	5″-TCTAGACGATCACGTTGATGGATCG-3′	ADE2 sense oligo nt 131–155
T7-141	5′-CCAAGCTTCTAATACGACTCACTATAGGGCCCCAATGTGTAACAAGTCATCGAC-3′	ADE2 antisense oligo nt 414–389 with T7 promoter (bold) and HindIII site (underlined)