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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: Nat Protoc. 2011 Feb 10;6(3):253–269. doi: 10.1038/nprot.2010.176

Figure 2.

Figure 2

Chromatograms for plasma immunodepletion and 2D HPLC chromatography. (a) Immunodepletion chromatogram of 300 μl of human plasma (see Box 1). (b) 2D HPLC chromatograms (2D RP chromatography). There is an eight-step elution for 1D AEX (see Box 2); protein fractions from each of the eight steps are further separated by 2D RP chromatography with a gradient elution (see Box 2).