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Effect of collision energy on collision-induced dissociation MS/MS analysis of transferrin glycopeptides Gp-1 and Gp-2. Blue squares represent GlcNAc. Blue squares with ″–pep″ represent the peptide with its core GlcNAc. [M-2Sia + H] + represents the protonated intact glycopeptide with loss of two sialic acids Gp-1: CGLVPVLAENYN*(5Hex4HexNAc2Sia)K; Gp-2: QQQHLFGSN*(5Hex4HexNAc2Sia)VTDCSGNFCLFR. CID, collision-induced dissociation.
