. Author manuscript; available in PMC: 2015 Dec 14.

Published in final edited form as: Nat Protoc. 2011 Feb 10;6(3):253–269. doi: 10.1038/nprot.2010.176

TABLE 1.

MALDI-DIT MS/MS deamination of a glycopeptide with an N-terminal glutamine as a function of collision energy.

Collision energy (V)	Peak intensities for glycopeptide		Ratio of peak intensity (P-17)/P	Peak intensities for GlcNac		Ratio of peak intensity (P-17)/P
Collision energy (V)	P	P-17	Ratio of peak intensity (P-17)/P	P	P-17	Ratio of peak intensity (P-17)/P
0.15	0.35	0.08	0.23	0.05	0.07	1.4
0.17	0.04	0.09	2.25	0.01	0.11	11
0.20	0.001	0.02	20	0.003	0.09	30
0.25	0.0008	0.018	22.5	0.002	0.05	25
0.30	0.0003	0.028	93.3	0.002	0.12	60

P: QQQHLFGSN*(5Hex4HexNAc2Sia)VTDCSGNFCLFR; P-17: QQQHLFGSN*(5Hex4HexNAc2Sia)VTDCSGNFCLFR – 17.