| • Step 1 (46 min) | Inject sample: 100% buffer A, 0.8 ml min−1 |
| • Step 2 (46 min) | 5% Mobile phase B, 0.8 ml min−1 |
| • Step 3 (46 min) | 10% Mobile phase B, 0.8 ml min−1 |
| • Step 4 (46 min) | 15% Mobile phase B, 0.8 ml min−1 |
| • Step 5 (46 min) | 20% Mobile phase B, 0.8 ml min−1 |
| • Step 6 (46 min) | 30% Mobile phase B, 0.8 ml min−1 |
| • Step 7 (46 min) | 50% Mobile phase B, 0.8 ml min−1 |
| • Step 8 (46 min) | 100% Mobile phase B, 0.8 ml min−1 |
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