Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications

. Author manuscript; available in PMC: 2015 Dec 14.

Published in final edited form as: Nat Protoc. 2011 Feb 10;6(3):253–269. doi: 10.1038/nprot.2010.176

• 0.00 min	5% Buffer B, 2.1 ml min⁻¹
• 5.00 min	5% Buffer B, 2.1 ml min⁻¹ (for desalting)
• 10.0 min	Start to collect fractions: 5% buffer B, 2.1 ml min⁻¹, 3 fractions per min, 0.7 ml per fraction
• 12.0 min	20% Buffer B, 2.1 ml min⁻¹
• 30.0 min	65% Buffer B, 2.1 ml min⁻¹
• 32.0 min	65% Buffer B, 2.1 ml min⁻¹
• 33.0 min	90% Buffer B, 2.1 ml min⁻¹
• 35.0 min	90% Buffer B, 2.1 ml min⁻¹
• 35.0 min	100% Buffer B, 3.0 ml min⁻¹
• 37.0 min	5% Buffer B, 2.1 ml min⁻¹
• 40.0 min	Stop to collect fractions: 5% buffer B, 2.1 ml min⁻¹
• 46.0 min	5% Buffer B, 2.1 ml min⁻¹