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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: Nat Protoc. 2011 Feb 10;6(3):253–269. doi: 10.1038/nprot.2010.176
• 0.00 min Inject sample, 100% buffer A, 0.5 ml min−1
• 6.30 min Start to collect flow-through fraction (low-abundance proteins): 100% Buffer A, 0.5 ml min−1
• 25.0 min 100% Buffer A, 0.5 ml min−1
• 25.1 min 100% Buffer B, 0.5 ml min−1
• 26.3 min Stop to collect flow-through fraction: 100% buffer B, 0.5 ml min−1
• 27.0 min 100% Buffer B, 3.0 ml min−1
• 29.0 min Start to collect bound fraction (high-abundance proteins): 100% Buffer B, 3.0 ml min−1
• 33.0 min Stop to collect bound fraction: 100% buffer B, 3.0 ml min−1
• 35.0 min 100% Buffer B, 3.0 ml min−1
• 35.1 min 100% Buffer A, 3.0 ml min−1
• 45.0 min 100% Buffer A, 3.0 ml min−1