Table 1.
Carbon (C) and nitrogen (N) content in percentage dry weight, C:N ratio and epiphyte cover of the leaves of E. acroides, the number of bacterial and eukaryotic OTUs obtained through ARISA and amplicon sequencing (bacteria: 16S rRNA gene, Illumina sequencing; eukaryotes: 18S rRNA gene, 454 sequencing); values constitute mean ± standard error where applicable; for the bacterial sequencing data set Chao1 richness estimates are given in italics with 95% confidence intervals in brackets
| ARISA | Amplicon sequencing | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| N [%] | C [%] | C : N ratio | Epiphyte cover [%] | Bacteria | Eukaryotes | Bacteria (16S) | Eukaryotes (18S) | ||
| Control | All ages | 1.42 ± 0.08 | 28.87 ± 0.8 | 20.98 ± 0.71 | 18.08 ± 2.45 | 96.52 ± 3.27 | 86.14 ± 2.56 | 507.5 ± 31.45 | 520.75 ± 84.25 |
| 917.31 ± 49.13 | |||||||||
| Youngest | 1.98 ± 0.18 | 34.63 ± 2.21 | 17.89 ± 2.5 | 3.25 ± 3.25 | 111.25 ± 6.02 | 72.5 ± 3.8 | 585 | 277 | |
| 913.76 (822.52/1040.05) | |||||||||
| Second youngest | 1.54 ± 0.1 | 29.68 ± 0.73 | 19.56 ± 0.88 | 14.3 ± 5.5 | 96.83 ± 7.49 | 86.67 ± 4.97 | 532 | 664 | |
| 991.53(863.52/1168.96) | |||||||||
| Third youngest | 1.2 ± 0.04 | 28.07 ± 0.8 | 23.47 ± 0.42 | 22.33 ± 2.72 | 94.83 ± 2.4 | 93.17 ± 3.36 | 462 | 561 | |
| 984.34 (827.90/1207.66) | |||||||||
| Oldest | 1.19 ± 0.04 | 25.83 ± 0.89 | 21.87 ± 1.31 | 22.54 ± 3.56 | 86.4 ± 6.31 | 88 ± 4.69 | 451 | 581 | |
| 779.63 (680.69 /921.19) | |||||||||
| Vent | All ages | 1.41 ± 0.12 | 26.93 ± 1.8 | 19.96 ± 0.79 | 6.61 ± 1.6 | 135.95 ± 2.82 | 89.68 ± 2.71 | 619.67 ± 48.22 | 517.75 ± 23.24 |
| 1044.09 ± 187.94 | |||||||||
| Youngest | 2.05 ± 0.05 | 32.02 ± 0.42 | 15.68 ± 0.42 | 2.5 ± 1.5 | 139.6 ± 7.64 | 97.4 ± 7.06 | 594 | 459 | |
| 933.61 (839.61/1063.58) | |||||||||
| Second youngest | 1.25 ± 0.21 | 24.87 ± 3.83 | 20.15 ± 0.74 | 8.7 ± 2.2 | 133.5 ± 5.94 | 87.33 ± 4.36 | NA | 553 | |
| Third youngest | 1.07 ± 0.18 | 24.26 ± 3.91 | 22.65 ± 1.3 | 8.55 ± 3.84 | 133.83 ± 2.7 | 84.33 ± 3.19 | 552 | 502 | |
| 788.18 (718.60 /886.80) | |||||||||
| Oldest | 1.29 ± 0.04 | 28.36 ± 0.1 | 22.04 ± 0.76 | 3.75 ± 0.75 | 140.5 ± 7.5 | 93.5 ± 7.5 | 713 | 557 | |
| 1410.49 (1240.43/1635.38) | |||||||||
| Total | 1.41 ± 0.07 | 27.92 ± 0.97 | 20.48 ± 0.53 | 12.81 ± 1.77 | 408 | 329 | 2179 | 3928 | |
| 3811.43 ± 142.83 | |||||||||