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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: Dev Dyn. 2010 Mar;239(3):855–864. doi: 10.1002/dvdy.22220

Figure 2. Development of hepatocyte canaliculi and intrahepatic biliary network.

Figure 2

Developmental pattern of the 2F11 epitope (A–E) and Mdr epitope, a canalicular transporter (A′–E′) with overlap of the two markers (A″–E″). The length and number of the canaliculi increases between 60 hpf and 120 hpf. The overlap of the two markers shows that each canaliculus develops in close association with the 2F11 positive biliary epithelia. Each canaliculus drains into a single intrahepatic bile duct. The ducts associated with many of the canaliculi in the 120 hpf sample are out of the plane of focus. (F, F′ and F″) Comparable confocal projections through the liver of a larva immunostained with the keratin-18 and Mdr antibodies. Note overlap (yellow) of the keratin-18 epitope in the terminal ductules with the Mdr protein in the canalicular membrane.