Figure 4. Liver cells respond to the Notch signal throughout larval development.

(A–F) Biliary expansion resumes upon reinitiation of Notch signaling associated with DAPT withdrawal. There is progressive increase in the complexity of the biliary network in 125 hpf larvae treated with DAPT for shorter intervals beginning at 53 hpf. All images are derived from larvae immunostained with the anti keratin-18 antibody. The gallbladder (g) is out of the plane of focus in panels D and E. Pancreas – p.