Figure 6.

dPCD marker genes are not up-regulated during HR PCD. qRT-PCR of Col-0 wild-type plants inoculated with an avirulent HR-inducing P. syringae strain in a time course experiment after infection. Relative expression of the indicated genes both in the inoculated area and in noninoculated tissue was determined by qRT-PCR at the indicated time points. PATHOGENESIS RELATED1 (PR1), MC1, and MYB DOMAIN PROTEIN30 (MYB30) were used as HR marker genes. Expression values were normalized using the SAND family gene as internal standard. Ratios of the expression values for each gene in the inoculated zone with respect to the noninoculated area are presented for each time point. Mean and se of the mean values were calculated from three independent experiments with three replicates. Statistical significance according to a Student’s t test P value of 0.005 is indicated by asterisks. hpi, Hours after inoculation; a.u., arbitrary units.