Table I. Analytical data on the Arabidopsis and poplar samples.
The AcBr and Klason lignin values represent the averages of two technical replicates ± sem. The ranges indicate the ranges of conjugate levels released from three independent lines, representing three biological replicates, each with two technical replicates. N/D, Not detected; —, assay was not performed.
| Method/Component | Wild-Type Arabidopsis | PMT Arabidopsis | Wild-Type Poplar | PMT Poplar | Maize (Rind) |
|---|---|---|---|---|---|
| AcBr lignin (wt% WCW) | 9.7 ± 0.8 | 9.7 ± 0.7 | 15.7 ± 1.7 | 15.0 ± 0.5 | 21.4 ± 0.5 |
| Klason lignin (wt% WCW) | — | — | 21.3 ± 0.7 | 21.5 ± 1.1 | — |
| Saponification | |||||
| p-Coumaric acid (mg g−1 WCW) | N/D | 1.0–2.0 | N/D | 1.2–3.5 | 39.6a |
| AcBr lignin (mg g−1 calculated) | — | 9.9–21 | — | 8.1–23 | 185 |
| Klason lignin (mg g−1 calculated) | — | — | — | 5.6–16 | — |
| p-Hydroxybenzoic acid (mg g−1 WCW) | N/D | N/D | 1.2 | 1.1–1.8 | N/D |
| AcBr lignin (mg g−1 calculated) | — | — | 7.6 | 7.5–12 | — |
| Klason lignin (mg g−1 calculated) | — | — | 5.6 | 5.1–8.4 | — |
| DFRC | |||||
| pCA (mg g−1 sample) | N/D | 1.1–1.7 | N/D | 0.9–2.0 | 4.8 |
| AcBr lignin (mg g−1) | — | 11–18 | — | 6–13 | 36.9 |
| Klason lignin (mg g−1 calculated) | — | — | — | 4–8 | — |
a
Maize has a large amount of arabinose-bound pCA that is released during saponification.