Genetic differences between R3 (MT+) and CJU10 (MT−) parental strains. A, Genetic differences, categorized by variant type (single-nucleotide variants [SNVs], insertions, and deletions), are shown with the total number of variant loci and total bases. B, Large-scale view of chromosomes 6 and 17, where 98.5% of the identified variants are located. Locations of sequence differences are shown in red. The region of chromosome 6 containing the mating locus is denoted by the blue bar. C, Schematic representation of the antibiotic resistance transgene insertion in CJU10 cpDNA. The aadA gene, along with the rbcL 5′ promoter and the psbC 3′ untranslated region (UTR), is inserted between inverted repeat region B (IRB) and the endogenous atpB gene.