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. 2015 Oct 14;169(4):2608–2623. doi: 10.1104/pp.15.00904

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Figure 6. — Effects of excess Fe on expression of AUX1 and PIN2 in Arabidopsis. Five-day-old wild-type seedlings, containing ProAUX1:AUX1-YFP, ProPIN2:PIN2-GFP, or ProPIN2:GUS constructs, were transferred onto control medium (Ctrl) or medium supplemented with 250 μm Fe (+Fe) at varying treatment times. The fluorescence signal was detected using a Zeiss LSM710 confocal laser-scanning microscope at the specified time points. One representative image for each experiment is shown. A, Effects of excess Fe on the expression of ProAUX1:AUX1-YFP and ProPIN2:PIN2-GFP in roots. The images were captured using the same confocal setting and are representative of at least 20 roots obtained from at least three independent experiments. B, The expression of ProPIN2:GUS with and without excess Fe for the indicated time periods. Bars = 50 μm (A) and 100 μm (B).