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. 2015 Oct 14;169(4):2608–2623. doi: 10.1104/pp.15.00904

Figure 9.

Figure 9.

Effects of AVG on the expression of AUX1 and PIN2 in Arabidopsis. Five-day-old wild-type seedlings, containing ProAUX1:AUX1-YFP or ProPIN2:PIN2-GFP constructs, were transferred onto control medium (Ctrl) or medium supplemented with 250 μm Fe (+Fe) with or without 1 μm AVG for 48 h. A, The expression of ProPIN2:PIN2-GFP with and without excess Fe plus AVG or Fe alone treatment. The treatments were maintained under the same confocal setting, and the inserts (a and b) show roots under Fe treatment, extending the master gain of the GFP channel from 679 to 1,017. B, The fluorescence of PIN2-GFP in A. Fluorescence is expressed relative to that of the control without AVG. Error bars represent se (n ≥ 5). C, The expression of ProAUX1:AUX1-YFP with and without excess Fe plus AVG or Fe alone treatment. The treatments were maintained under the same confocal setting, and the inserts (a–d) show images of partial enlargement, as indicated by white arrows. D, The fluorescence of AUX1-YFP in C. Fluorescence is expressed relative to that of the control without AVG. Bars represent se (n ≥ 5). E, Effect of excess Fe (250 μm) on LR initiation events in wild-type (Col-0), eto1-1, aux1-7, and eto1/aux1 seedlings after 5 d. Values are the means ± se (n ≥ 7). Bars = 50 μm.