Table II. Summary of venation pattern results for traits D_V (vein density; millimeters millimeters⁻²), D_BP (branching point density; millimeters⁻²), D_EP (ending point index; millimeters⁻²), and D_AR (areole no. density; millimeters⁻²).

Values are mean values ± sd (n = 10).

Leaf Type and Trait	Col-0	hve-2	Ler-0	ond3	as2-101
Cotyledons
DV	1.60 ± 0.08	1.41 ± 0.13a	1.48 ± 0.12	1.39 ± 0.08	1.36 ± 0.05a
DBP	0.71 ± 0.16	0.56 ± 0.11a	0.72 ± 0.20	0.68 ± 0.12	0.58 ± 0.05
DEP	0.20 ± 0.14	0.14 ± 0.11	0.15 ± 0.11	0.13 ± 0.11	0.17 ± 0.10
DAR	0.47 ± 0.09	0.35 ± 0.09a	0.45 ± 0.12	0.42 ± 0.09	0.39 ± 0.05
Leaves 1 + 2
DV	2.92 ± 0.14	2.06 ± 0.07a	2.87 ± 0.14	3.56 ± 0.21a	3.25 ± 0.21a
DBP	3.39 ± 0.33	1.60 ± 0.15a	3.58 ± 0.38	6.35 ± 0.74a	4.37 ± 0.64a
DEP	0.87 ± 0.27	0.76 ± 0.13	0.95 ± 0.18	1.84 ± 0.25a	1.29 ± 0.18a
DAR	1.32 ± 0.18	0.47 ± 0.05a	1.37 ± 0.17	2.37 ± 0.40a	1.59 ± 0.30
Leaf 6
DV	2.61 ± 0.38	2.17 ± 0.29a	2.64 ± 0.39	4.37 ± 0.34a	2.80 ± 0.35
DBP	2.67 ± 0.93	1.84 ± 0.42a	3.08 ± 0.98	8.70 ± 1.45a	3.17 ± 0.82
DEP	0.94 ± 0.26	0.81 ± 0.18	0.82 ± 0.2	1.75 ± 0.13a	1.19 ± 0.19a,b
DAR	0.92 ± 0.38	0.56 ± 0.16a	1.15 ± 0.41	3.53 ± 0.74a	1.03 ± 0.39

^aStatistically significant differences (P < 0.05) comparing a mutant line with its corresponding wild type: hve-2 versus Col-0 and ond3/as2-101 versus Ler-0 (standard Student’s t test).

^bSome of the as2-101 leaves 6 were cut to allow flat imaging. For this reason, the number of free ending points N_EP found by phenoVein was increased inadvertently by the cut veins. These cases were corrected manually by subtracting the undesired ending points. The complete data set containing the results of phenoVein are listed in Supplemental Table S1.