Changes in the metabolite levels in shoots and roots of tomato plants subjected to varying nutritional stress. Differences in metabolite abundance on a fresh weight basis were calculated by dividing the metabolite levels in the plants subjected to nutritional stress (Fe deficiency [F], S deficiency [S], and dual deficiency [D]) by the levels in the control plants (C). Blue and red represent decreases and increases, respectively, compared with C plants. Fold changes of NA and 4-hydroxy-Pro in roots between two conditions were calculated and colored orange, because the metabolites were not at detectable levels in other two conditions. Data of ethylene production in roots were published (Zuchi et al., 2009). AAA, Aromatic amino acid; BCAA, branched chain amino acid; GABA, γ-aminobutyric acid; na, not analyzed; nd, not detected (below detection limit); TCA, tricarboxylic acid.