Figure 8.

OsWRKY53 negatively regulates TrypPI production and resistance of rice to the SSB. A and B, Mean TrypPI activities (+se, n = 5) in ir-wrky and oe-WRKY lines and wild-type (WT) plants that were individually infested by a third-instar SSB larva for 3 d. C and D, Mean larval mass (+se, n = 60) of SSB that fed on ir-wrky and oe-WRKY lines or wild-type plants for 12 d. E, Mean activities (+se, n = 5) of TrypPIs in oe-WRKY lines and wild-type plants that were individually treated with 100 μg of MeJA in 20 μL of lanolin paste (MeJA) or with 20 μL of pure lanolin (insert), followed by a SSB larva feeding for 3 d. F, Mean increased larval mass (+se, n = 60) of SSB larvae 12 d after they fed on oe-WRKY lines and wild-type plants that were individually treated with 100 μg of MeJA in 20 μL of lanolin paste (MeJA) or with 20 μL of pure lanolin (insert). G, Damaged phenotypes of ir-wrky and oe-WRKY lines and wild-type plants that were individually infested by a third-instar SSB larva for 14 or 7 d (n = 20). Letters indicate significant differences between lines (P < 0.05, Duncan’s multiple range test). Asterisks indicate significant differences in ir-wrky and oe-WRKY lines compared with wild-type plants (*, P < 0.05; and **, P < 0.01, Student’s t tests). Con, Control plants.