Figure 3.

Nodulation phenotypes of AeCCaMK knockdown mutants. A, Percentage of nodulated roots calculated at 7 and 18 dpi with the Bradyrhizobium spp. ORS278 strain (the control corresponds to transgenic roots transformed with an empty vector; transgenic control [n = 27], RNAiCCaMK1 [n = 84], and RNAiCCaMK2 [n = 76]). Different letters above the bars represent statistically significant differences (χ² test, P < 0.05). B to D, Transgenic control nodule. E to I, RNAiCCaMK2 at 18 dpi. D to G are longitudinal sections (60 µm) of nodules. Bars = 500 µm. J, Quantification of AeCCaMK mRNA levels in RNAi plants determined by real-time quantitative PCR. Quantification was performed on five independent RNAiCCaMK2 lines. AeEF1α was used as a reference gene. Relative expression levels of AeCCaMK in three independent transgenic control roots are shown. Expression levels are relative to transgenic control line 5.