Figure 7.

Colonization levels in M. truncatula rad1. A, Quantitation of total infection units, with hyphopodia only, infection units with intercellular hyphae only, and infection units with arbuscules in rad1 and a wild-type segregant (WT-seg) from the rad1 backcrossed population at 2 and 6 dpi with G. versiforme in a double-cone assay system. For each line, the data are the means of three biological replicate cones each containing two plants. B, The total percentage of colonized root length in rad1 and R108 at 3 and 5 wpp with G. versiforme. Plants were fertilized with a modified one-half-strength Hoagland’s solution with 20 µm phosphate. C, The total percentage of colonized root length in rad1 and R108 at 3, 5, and 7 wpp with G. versiforme. Plants were fertilized with a modified one-half-strength Hoagland’s solution with 200 µm phosphate. Error bars show sem (n ≥ 3). Asterisks indicate significant differences (Student’s t test). *, P < 0.05; **, P < 0.01.