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. 2015 Oct 26;169(4):2848–2862. doi: 10.1104/pp.15.01170

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© 2015 American Society of Plant Biologists. All Rights Reserved.

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Figure 6. — Analyses of starch, sugar, nitrogen, and gene expression involved in carbon metabolism and carbon and nitrogen transport in flag leaves of wild-type (WT) and pPP2::AtSUC2 rice plants. A, Leaf starch levels (n = 5). B. Leaf Suc levels (n = 5). C, Leaf Fru levels (n = 5). D, Leaf Glc levels (n = 5). DW, Dry weight. E, Analysis of genes involved in starch biosynthesis in flag leaves. F, Analysis of genes involved in Suc biosynthesis in flag leaves. G, Analysis of genes involved in carbon transport in flag leaves. H, Total nitrogen (n = 6). I, Soluble protein content (n = 6). J, Analysis of genes involved in nitrogen transport in flag leaves. Analyses were performed on the flag leaves on the 10th day after fertilization. All data are given as the mean ± sd. Asterisks indicate significant difference from the wild type (**, P < 0.01). A Student’s t test was used to generate the P values. For the analysis of gene expression, real-time PCR was performed, and five independent experiments were done for each line using Actin (Os01g0376700) as control gene. Shown is the fold change in gene expression relative to the wild type. Starch and sugar were determined at the end of day (ED) and end of night (EN), respectively. BEI (Os06g0726400), BEIIa (Os02g0528200), and BEIIb (Os02g0528200), encoding for starch-branching enzymes; AGPL1 (Os05g0580000), AGPL2 (Os01g0633100), AGPL3 (Os03g0735000), AGPL4 (Os07g0243200), AGPS1 (Os09g0298200), AGPS2a (Os08g0345800), and AGPS2b (Os08g0345800), encoding for ADP-Glc pyrophosphorylase subunits; SSI (Os06g0160700), SSIIa (Os06g0229800), SSIIb (Os02g0744700), SSIIc (Os10g0437600), SSIIIa (Os08g0191500), SSIIIb (Os04g0624600), SSIVa (Os01g0720600), and SSIVb (Os05g0533600), encoding for starch synthase; GBSSI (Os06g0133000) and GBSSII (Os07g0412100), encoding for granule-bound starch synthases; ISA2 (Os05g0395300) and ISA3 (Os09g0468700), encoding for isoamylases; SPP (Os01g0376700), encoding for Suc-phosphatase; UGP1 (Os01g0264100) and UGP2 (Os02g0117700), encoding for UDP-Glc pyrophosphorylases; SPS1 (Os01g0702900), SPS2 (Os02g0184400), and SPS6 (Os06g0634800), encoding for Suc-phosphate synthases; cyFBP1 (Os01g0866400), encoding for Fru-1,6-bisphosphatase; PFPɑ (Os02g0714200), encoding for pyrophosphate-Fru 6-phosphate 1-phosphotransferase; PPase (Os01g0866500), encoding for pyrophosphatase.