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. 2015 Oct 26;169(4):2848–2862. doi: 10.1104/pp.15.01170

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© 2015 American Society of Plant Biologists. All Rights Reserved.

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Figure 7. — Analyses of total carbon and expression levels of genes involved in carbon metabolism and transport in seeds of wild-type (WT) and pPP2::AtSUC2 rice plants. A, Total carbon levels (percentage) in dry seeds (n = 5). B, Analysis of genes involved in carbon transport in developing seeds on the 10th day after fertilization. C, Analysis of genes involved in starch biosynthesis in developing seeds on the 10th day after fertilization. All data are given as the mean ± sd. Asterisks indicate significant difference from the wild type (**, P < 0.01). A Student’s t test was used to generate the P values. For the analysis of gene expression, real-time PCR was performed, and five independent experiments were done for each line using Actin (Os01g0376700) as control gene. Shown is the fold change in gene expression relative to the wild type. BEI (Os06g0726400), BEIIa (Os02g0528200), and BEIIb (Os02g0528200), encoding for starch-branching enzymes; AGPL1 (Os05g0580000), AGPL2 (Os01g0633100), AGPL3 (Os03g0735000), AGPL4 (Os07g0243200), AGPS1 (Os09g0298200), AGPS2a (Os08g0345800), and AGPS2b (Os08g0345800), encoding for ADP-Glc pyrophosphorylase subunits; SSI (Os06g0160700), SSIIa (Os06g0229800), SSIIb (Os02g0744700), SSIIc (Os10g0437600), SSIIIa (Os08g0191500), SSIIIb (Os04g0624600), SSIVa (Os01g0720600), and SSIVb (Os05g0533600), encoding for starch synthase; GBSSI (Os06g0133000) and GBSSII (Os07g0412100), encoding for granule-bound starch synthases; ISA2 (Os05g0395300) and ISA3 (Os09g0468700), encoding for isoamylases. Susy1 (Os06g0194900), Susy2 (Os03g0401300), and Susy3 (Os07g0616800), encoding for Suc synthases; PPDKB (Os05g0405000), encoding for pyruvate phosphate dikinase; PGIa (Os09g0465600) and PGIb (Os06g0256500), encoding for phosphoglucose isomerases; Amy3B (Os09g0457500), Amy3C (Os09g0457800), Amy3D (Os08g0473900), and Amy3E (Os08g0473600), encoding for alpha-amylase isozymes.