Table I. Plant phenotype of control and transgenic OsMADS26 lines after 7 d of in vitro culture (MS), 72 DAG in greenhouse, and from flowering to harvest.
Results shown are from one of two independent biological repetitions that produced similar results. Reported values are the means and se for three individual plants of each line. A Student’s t test was done to establish whether the parameter measured in transgenic lines was different from corresponding control lines. BEG, Flowering beginning; DW, plant dry weight after seed harvesting; FD, flowering date; HTG_7, plant height measured at 7 DAG; HTG_76, plant height measured at 76 DAG; P1,000:, weight of 1,000 seeds; PW, panicle weight; TIL_76, number of tillers counted at 76 DAG. Significant difference: *, P < 0.05, **, P < 0.01; and ***, P < 0.001.
Line name | HTG_7 | HTG_76 | TIL_76 | BEG | FD | DW | PW | P1,000 |
---|---|---|---|---|---|---|---|---|
cm | DAG | g | ||||||
Wild type | 6.06 ± 1.51 | 97.53 ± 0.59 | 12.33 ± 0.33 | 80.33 ± 0.33 | 81.67 ± 0.88 | 9.74 ± 2.34 | 15.18 ± 2.45 | 21.80 ± 0.71 |
OX0 | 6.34 ± 1.33 | 97.47 ± 2.06 | 10.67 ± 0.33 | 82.33 ± 1.20 | 84.00 ± 1.00 | 8.73 ± 0.87 | 9.52 ± 0.95 | 20.34 ± 0.62 |
DR0 | 6.72 ± 1.27 | 95.23 ± 1.36 | 11.33 ± 1.33 | 81.67 ± 0.67 | 83.67 ± 0.67 | 7.96 ± 3.80 | 8.88 ± 4.28 | 17.89 ± 3.93 |
OX1 | 3.84 ± 0.67** | 100.60 ± 2.17 | 10.33 ± 1.45 | 80.00 ± 1.53 | 81.67 ± 1.86 | 8.00 ± 1.42 | 8.78 ± 1.50 | 21.39 ± 0.30 |
OX2 | 2.41 ± 0.92*** | 93.40 ± 2.84 | 12.33 ± 0.88 | 83.67 ± 0.88 | 86.00 ± 1.00 | 8.21 ± 1.12 | 8.93 ± 1.34 | 20.38 ± 0.72 |
DR5-1 | 1.68 ± 0.68*** | 87.90 ± 2.51* | 7.80 ± 2.08 | 83.00 ± 1.15 | 85.67 ± 0.67 | 3.86 ± 1.07 | 4.21 ± 1.14 | 16.32 ± 0.48 |
DR5-2 | 1.61 ± 0.29*** | 95.37 ± 1.84 | 6.67 ± 0.67* | 82.33 ± 0.67 | 85.00 ± 0.00 | 4.93 ± 0.40 | 5.48 ± 0.39 | 19.79 ± 1.15 |
DR3-1 | 1.61 ± 0.31*** | 90.53 ± 1.79 | 9.67 ± 1.33 | 85.00 ± 0.00** | 87.00 ± 0.58** | 6.62 ± 1.37 | 7.33 ± 1.65 | 21.42 ± 0.73 |
DR3-2 | 0.84 ± 0.18*** | 97.20 ± 1.73 | 9.00 ± 1.00 | 84.67 ± 0.33** | 86.33 ± 0.33* | 7.76 ± 0.73 | 8.41 ± 0.67 | 20.01 ± 0.68 |