Overexpression of PpPTEN in a wild-type (WT) background. A, One-millimeter pieces of protonemata tissue of the wild type (WT) and PpPTENA, PpPTENB, and PpPTEND overexpression lines grown on minimal media for 4 weeks. B, Number of gametophores of the wild type and pten overexpressor lines at 1, 2, and 3 weeks of growth on minimal media. Values represent mean ± sd. Asterisks indicate values that are significantly different from the wild type (*P < 0.05 and **P < 0.01, Students t test). C, Rhizoid length of the wild type and pten overexpressor lines. Values represent mean ± sd (n ≥ 18). Asterisks indicate values that are significantly different from the wild type (P < 0.05, Student’s t test). D, Rhizoid cell length of the wild type and pten overexpressor lines. Values represent mean ± sd (n ≥ 30). Bar = 0.2 cm.