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CIPK23 phosphorylates HAK5 in vitro. GST fusions encompassing the HAK5 N-terminal region (NT), the internal loop (IL), or the cytosolic tail (CT) were subjected to phosphorylation by CIPK23 and resolved by SDS/PAGE. A, Coomassie Brilliant Blue staining of gel showing proteins in the assay. B, Autoradiograph showing incorporation of 32P into the N-terminal region of HAK5.
