Figure 3.

Phosphorylation kinetics upon a switch from far-red to blue light. Total protein was extracted from 15-d-old seedlings (three per time point) that were exposed to far-red light for 1 h and then switched to blue light. The level of phosphorylation was determined at different times after the switch (time 0) to blue light. A, Immunodetection of Lhcb1. B, Immunodetection of Lhcb2. C, Phosphorylation levels for Lhcb1 (dotted black line) and Lhcb2 (plain gray lines) of five samples, such as those presented in A and B, are plotted as a function of time. The lines represent a sigmoidal fit of the data. Triangles, squares, and circles represent distinct biological replicates of the experiment. D, The same data as in C are normalized to the final phosphorylation level (phosphorylation at t = 3,600 s equals 100%). P and U indicate the phosphorylated and unphosphorylated forms, respectively.