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. 2015 Oct 15;26(1):427–439. doi: 10.1093/cercor/bhv232

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© The Author 2015. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Signaling mechanism underlying the NgR1-mediated restriction of synaptic AMPAR delivery in the adult barrel cortex. (A) (Left) Synaptic responses from shTROY-expressing (Sh TROY) and scrambled control-shRNA-expressing (Scramble) neurons at layer 4–2/3 of WT adult barrel cortex. Scale bars: 50 pA, 20 ms. (Right) Mean A/N ratio. Scramble: n = 5 (10 cells from 5 animals); ShTROY: n = 6 (11 cells from 6 animals). *P < 0.05. (B) (Left) Synaptic responses from shTROY-expressing (Sh TROY) and scrambled control-shRNA-expressing (Scramble) neurons at layer 4–2/3 synapses of NgR1-deficient adult barrel cortex. Scale bars: 50 pA, 20 ms. (Right) Mean A/N ratio. Scramble: n = 5 (10 cells from 5 animals); ShTROY: n = 5 (11 cells from 5 animals). (C) (Left) Synaptic responses from shTROY-expressing (Sh TROY) and shTROY+shRNA-resistant TROY (res-TROY) neurons at layer 4–2/3 synapses of the WT adult barrel cortex. Scale bars: 50 pA, 20 ms. (Right) Mean A/N ratio. ShTROY: n = 5 (6 cells from 5 animals), ShTROY+ res-TROY: n = 5 (6 cells from 5 animals). *P < 0.05. (D) Phosphorylation level of ADF/cofilin in the synaptoneurosome fraction from the adult barrel cortex of WT and NgR1-deficient (KO) mice. The total ADF/cofilin level was used as the reference in the quantitative analysis. The ADF/cofilin phosphorylation level in KO mice was normalized to that of WT. WT: n = 10; KO: n = 11. *P < 0.05. (E) (Left) Synaptic responses from Venus-tagged dominant-negative ADF/cofilin-expressing (Cofilin S3E-venus) and venus-expressing (Venus) neurons at layer 4-2/3 synapses of the NgR1-deficient adult barrel cortex. Scale bars: 50 pA, 20 ms. (Right) Mean A/N ratio. Cofilin S3E: n = 4 (9 cells from 4 animals); Venus: n = 5 (13 cells from 5 animals, same data in Fig. 2G). *P < 0.05. (F) (Left) Synaptic responses from Venus-tagged dominant-negative ADF/cofilin-expressing (Cofilin S3E-venus) and venus-expressing (Venus) neurons at layer 4-2/3 synapses of the WT adult barrel cortex. Scale bars: 50 pA, 20 ms. (Right) Mean A/N ratio. Cofilin S3E: n = 5 (11 cells from 5 animals); Venus: n = 5 (9 cells from 5 animals). Data were analyzed by Student's t-test. Data are presented as mean ± SEM.