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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Kidney Int. 2015 Mar 18;88(3):490–502. doi: 10.1038/ki.2015.73

Figure 5.

Figure 5

Notch regulates SMC migration and neointima formation in AVFs. A. TGF-β1 regulated out-growth of SMCs was Notch-dependent: artery fragments from WT and RBP-Jκ KO mice were seeded into DMEM media-filled plates. TGF-β1 was added, after 7 days, outgrowing SMCs were counted (Representative data from 3 animals). B. Inducible KO of RBP-Jκ in SMCs of AVFs. AVFs from RBP-Jκflox/flox/SMMHC-CreER2+ mice were treated with or without tamoxifen, RBP-Jκ and FSP-1 expression in AVFs were detected by immunostaining. Tamoxifen-treated mice had virtually no RBP-Jκ expression in neointima cells. C. Trichrome staining of of AVFs created in Control and RBP-Jκ KO mice with CKD is shown. D. RBP-Jκ KO inhibits FSP-1 expression as detected by immunostaining in AVFs created in Control and RBP-Jκ KO mice with CKD. F. The ratio of lumen to neointima areas was improved in RBP-Jκ KO mice. (L, lumen). Scale: 50 μm.