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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Kidney Int. 2015 Mar 18;88(3):490–502. doi: 10.1038/ki.2015.73

Figure 6.

Figure 6

FSP-1 KO suppresses SMC migration and CKD-induced neointima formation. A. Common carotid arteries from WT or FSP-1 KO mice were cut into small pieces and cultured in DMEM. Pieces of arteries from WT or FSP-1 KO mice were infected with full length AdJagged1 or control adenovirus on day 2. SMC outgrowth was determined after 7 days. Representative data from two repeated experiments are shown. B. Jagged1 expression induced FSP-1 expression. SMCs from WT or FSP-1 KO mice were infected with a Jagged1 adenovirus and FSP1 was detected. C. FSP-1 KO inhibits SMC outgrowth. Pieces of common carotid arteries from WT or FSP-1 KO mice were cultured in DMEM. They were infected with AdFSP-1 or control adenovirus on day 2. SMC outgrowth was determined after 3 and 7 days (Scale: 50 μm). D. The number of outgrowing cells in a total of 3 views from 3 samples in each group was counted. E. In mice with CKD, the ratio of the lumen to neointima areas in AVFs from FSP-1 KO mice were compared to values from WT mice (mean ± SE, n = 5).