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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Kidney Int. 2015 Mar 18;88(3):490–502. doi: 10.1038/ki.2015.73

Figure 7.

Figure 7

In failed AVFs from ESRD patients, FSP-1 is expressed in neointima cells. A. Morphology and SMA-α-positive cells from failed AVFs of ESRD patients (n =5). H & E (left panel) and SMA-α immunostaining (right panel) are shown. B. Characterization of neointima cells found in AVFs from ESRD patients. Cross sections of AVFs were immunostained for the FSP-1, PCNA, and SMA-α. (Red, double headed arrows show the neointima thickness, and green arrows indicate the media of AVFs). C. Coimmunostaining of FSP-1 and SMA-α in AVFs revealed that FSP-1 positive cells also express SMA-α. D. Activated Notch (N1ICD, brown color) is mainly located in nuclei of neointima cells in the failed AVFs of ESRD patients. E. In AVFs from ESRD patients, SMCs expressing N1ICD co-stained positively for FSP-1. The expression of N1ICD (green) and FSP-1 (red) were determined by double immunostaining; the double-head arrow indicates the neointima (red) and the smooth muscle layer of the vein (green). The red arrow points to cells stained positively for N1ICD and FSP-1. (N, neointima; M, media; L, lumen). Scale: 50 μm. F. Staining for H & E (Left panel) and Masson Trichome (Right panel) of human PTFE grafts are shown. Representative pictures from two PTFE grafts are shown. G. Co-immunofluorescent of SMA-α and FSP-1 in PTFE graft revealed that FSP-1 positive cells also express SMA-α. H. In PTFE graft from ESRD patients, expression of N1ICD and FSP-1 was detected by immunofluorescent staining with FSP-1 (n = 7).