Table 5. Nano flow-rate reversed-phase HPLC gradient elution 300 nL/min.
| Time (min) | Mobile phase B (%) |
|---|---|
| 0.00 | 3.00 |
| 2.00 | 7.00 |
| 92.0 | 35.0 |
| 93.0 | 50.0 |
| 102.0 | 50.0 |
| 103.0 | 95.0 |
| 108.0 | 95.0 |
| 109.0 | 3.00 |
| 130.0 | 3.00 |
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