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. 2015 Dec 15;26(25):4541–4551. doi: 10.1091/mbc.E15-08-0548

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© 2015 Speldewinde et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — [PSI⁺] prions are formed in autophagy mutants. (A) Representative fluorescence micrographs for the wild-type and indicated autophagy mutant strains containing the Sup35NM-GFP plasmid. The Sup35NM-GFP plasmid was induced for 1 h using copper before visualizing aggregate formation after 16 h of growth. (B) The aggregation frequency was calculated in the indicated autophagy mutants as a percentage of the number of cells containing fluorescent foci out of ∼300 cells counted. (C) Representative fluorescence micrographs for strains cured with GdnHCl.