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. 2015 Dec 15;26(25):4541–4551. doi: 10.1091/mbc.E15-08-0548

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© 2015 Speldewinde et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Sup35 aggregate formation occurs at similar intracellular sites in wild-type and atg1 mutant strains. (A) The vacuolar dye FM4-64 was used to visualize vacuolar membranes in wild-type and atg1 mutant strains. Similar vacuolar Sup35-GFP foci were detected in the wild-type and atg1 mutant strains after copper induction of the SUP35NM-GFP fusion construct for 1 h. (B) CFP-ATG8 and CFP-ATG14 were used as markers of PAS in wild-type and atg1 mutant strains. Strains were grown for 48 h in the presence of 4 mM spermidine to induce autophagy and SUP35NM-GFP induced with copper for 1 h. Sup35 aggregates form adjacent to PAS markers in the wild-type strain.