FIGURE 3:

Inhibition of Drp1 diminishes radiation-induced mitochondrial fission. (A, B) WT and KO MEFs were irradiated at 10 Gy of x-rays and incubated for 12 h. (A) Representative confocal images of mitochondria in MEFs with or without x-irradiation. IR, ionizing radiation. (B) Quantitative analysis of mitochondrial morphologies in WT and KO MEFs. (C, D) After x-irradiation at 10 Gy in the presence or absence of 25 μM Mdivi-1, WT MEFs were incubated for 12 h. (C) Representative confocal images of mitochondria in WT MEFs in the presence or absence of Mdivi-1 with or without x-irradiation. (D) Quantitative analysis of mitochondrial morphologies in WT MEFs in the presence or absence of Mdivi-1. Quantitative image analysis of mitochondrial morphologies was performed as described in Materials and Methods. Data are expressed as means ± SD of three experiments.