Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 15;26(25):4607–4617. doi: 10.1091/mbc.E15-03-0181

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Yamamori et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 5: — The apoptosis pathway is not involved in radiation-induced MC. (A) Effect of a caspase inhibitor on radiation-induced MC. WT MEFs were x-irradiated at 10 Gy in the presence of pan-caspase inhibitor Z-VAD-FMK at 0 (black), 5 (white), or 20 (gray) μM. After 24 h of incubation, MC was scored as described in Materials and Methods. Data are expressed as means ± SD of three experiments. n.s., not significant. (B) Cytosolic release of mitochondrial proteins after x-irradiation. After x-irradiation at 10 Gy, WT MEFs were incubated for the indicated times. Cytosol and organelle fractions were collected, and subcellular distribution of cytochrome c (cyt c) and Smac was analyzed by Western blotting. Treatment with etoposide (Eto; 50 μM, 8 h) was used as positive control. GAPDH and VDAC were loading controls for the cytosol and organelle fraction, respectively. Representative blots of three experiments.