FIGURE 8:

Drp1 deficiency elevates cyclin B1 expression after irradiation. (A) The effect of Drp1 deficiency on protein expression profiles after irradiation. After WT or KO MEFs were x-irradiated at 10 Gy, the cells were collected at the indicated times. The expression of the indicated proteins was analyzed by Western blotting. Representative blots of three experiments. Actin was used as loading control. p-H3, phospho–histone H3. (B) Time-course change of cyclin B1 abundance after irradiation in WT and KO MEFs. WT (black) or KO (white) MEFs were x-irradiated at 10 Gy, and the expression of cyclin B1 was analyzed by Western blotting. The abundance of cyclin B1 in both types of MEFs at the indicated times was quantified relative to that in WT MEFs at 0 h. Data are expressed as means ± SD of three experiments. *p < 0.05, **p < 0.01 vs. WT MEFs (Student’s t test).