FIGURE 4:

Pib2 stimulates TORC1 signaling independent of the Ragulator/EGO complex. Strains bearing pib2∆, gtr1∆, gtr2∆, or ego1∆ knockout mutations (∆ symbol sometimes omitted) and also bearing either the TOR1-I1954S point mutation (A), the npr2∆ mutation (B), or plasmids expressing hyperactive (-h) and inactive (-i) variants of Gtr1 or Gtr2 (C) were grown in SC or SC-uracil medium, exposed to tunicamycin plus FK506 for 8 h, stained with PI, and analyzed by flow cytometry. The percentages of PI-positive (dead) cells were calculated from the average of three replicate cultures (± SD).